monoclonal cd86 (b7-2) pe  (Thermo Fisher)

Journal: Redox Biology

Article Title: Redox-modulating macrophage biohybrid nanoplatform for targeted RIPK1-PANoptosome suppression in ischemic stroke

doi: 10.1016/j.redox.2025.103997

Figure Lengend Snippet: In vitro evaluation of microglia regulation of MA@ULips. (a) Scheme of microglia phenotype transition induced by MA@ULips. (b) Flow cytometry analysis of CD86 and CD206 in BV2 cells after diverse treatments. Representative immunofluorescence staining images of (c) CD86 and (d) CD206 in BV2 cells after various treatments. The level of (e) Arg-1, (f) IL-10, (g) TNF-α, and (h) IL-6 in the BV2 cells after different treatments (n = 3). Data are presented as mean ± SD. Statistical significance was calculated by one-way ANOVA with Tukey's multiple comparisons test. ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Anti-caspase 1 (31020-1-AP), anti-RIPK1 (17519-1-AP) and PE- anti -mouse CD86 + (PE-65068) were obtained from Proteintech Group.

Techniques: In Vitro, Flow Cytometry, Immunofluorescence, Staining

Journal: Redox Biology

Article Title: Redox-modulating macrophage biohybrid nanoplatform for targeted RIPK1-PANoptosome suppression in ischemic stroke

doi: 10.1016/j.redox.2025.103997

Figure Lengend Snippet: MA@ULips enhancing neurological functional recovery and modulating ischemic inflammatory microenvironment. (a) Experimental design involved multiple functional tests to evaluate the neurological outcomes of MA@ULips. (b) Forelimb asymmetry rate assessed through the cylinder test (n = 8). (c) Motion paths in the open field test. (d) Quantitative analysis of movement distance (n = 7). (e) Representative images of sham-operated mice and MCAO/R mice after different treatments. (f) Neurological deficit evaluation by the mNSS (n = 6). (g) Immunohistochemical staining for GFAP on brain tissue from the ischemic cortex area after different treatments. (h) Immunofluorescent staining of brain tissue for Iba-1 and CD86 after different treatments. (i–j) The levels of (i) IL-10 and (j) IL-6 in serum of ischemic mice after different treatments (n = 3). (k–l) The levels of (k) IL-6 and (l) IL-10 in brain tissues of ischemic mice after different treatments (n = 3). Data are presented as mean ± SD. Statistical significance was calculated by one-way ANOVA with Tukey's multiple comparisons test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Anti-caspase 1 (31020-1-AP), anti-RIPK1 (17519-1-AP) and PE- anti -mouse CD86 + (PE-65068) were obtained from Proteintech Group.

Techniques: Functional Assay, Immunohistochemical staining, Staining

Journal: Regenerative Therapy

Article Title: Influence of extracorporeal shockwaves on macrophage polarization in a 3D collagen matrix

doi: 10.1016/j.reth.2025.06.003

Figure Lengend Snippet: Overview of MFI values of CD86 expression measured by flow cytometry. The MFI values of the CD86 antibody in flow cytometry are presented as a boxplot (median and interquartile range) and divided into four subgroups based on the type of treatment: “+ESWT” ( n = 10), “–ESWT“ ( n = 10), “Untreated” ( n = 9), and “Positive Control Gel” ( n = 6). Individual data points are represented as dots in the bar chart. Macrophages from the same patient are consistently color-coded across all images. No statistically significant differences could be observed between the groups (“–ESWT” vs. “+ESWT” : p = 0.912; “+ESWT” vs. “Untreated” : p = 0.154; “+ESWT” vs. “Positive Control Gel” : p = 0.780; “–ESWT” vs. “Untreated” : p = 0.178; “–ESWT” vs. “Positive Control Gel” : p = 0.695; “Untreated” vs. “Positive Control Gel” : p = 0.060).

Article Snippet: Monoclonal CD86 (B7-2) , PE , Thermo Fisher Scientific Inc., Waltham, MA, USA , M1-phenotype.

Techniques: Expressing, Flow Cytometry, Positive Control